Diaspro Alberto

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Alberto Diaspro is Director of the Department of Nanophysics at the Istituto Italiano di Tecnologia (IIT), Deputy Director of IIT, Chair of the Nikon IMaging Center at IIT (www.nic.iit.it). AD is Professor of Applied Physics at the Department of Physics of University of Genova and supervisor for the Ph.D. Courses in the Bioengineering and Robostics and Physics programs of the Universitry of Genova within the IIT program. He was President of OWLS (Optics with Life Sciences), EBSA (European Biophysical Societies Association) and Appointed Vice President of ICO (Interational Commission of Optics). AD is afounder of the Nanoscale Biophysics Subgroup of the Biophysical Society (*). During the 90’s he carried out part of his research activity at Drexel University (PA, USA), Universidad Autonoma de Madrid (Spain) and Czech Academy of Sciences (Czech Republic). He also coordinated a research program (2004-2012) at IFOM-IEO Campus in Milano on Biomedical Research and is currently associated with the Institute of Biophysics of the National Research Council (CNR)(since 2006). He founded LAMBS (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy) in 2003 - www.lambs.it. AD realized a hybrid artificial “nanobiorobot” within EU and national Research Projects (2000-2005), and designed and realized the first Italian multiphoton microscope within a research grant of the National Institute of Physics of Matter (1999). He directed the design and realization of the first Italian nanoscopy architecture at the Neuroscience and Brain Technologies Department of IIT (2008).

At present, Alberto Diaspro coordinates the Nanobiophotonics IIT research program (**). He is coordinator of several EU and national research programs, and published more than 300 international peer reviewed papers, 6000 citations, H=38 (source Google Scholar ***). He is EDitore in Chief of the international Journal MIcroscopy Research and Technique and active member of international editorial boards and societies (SIOF, SIF, SISM, SIBPA, BS, EBSA, OWLS, IEEE, SPIE, OSA). AD is IEEE senior member and SPIE fellow (http://spie.org/profile/Alberto.Diaspro-6137). AD He received the Emily M.Gray Award in 2014. (*V)

His specific research experience is related to the design, realization and utilization of optical and biophysical instrumentation as far-field super resolution optical microscopy and nanoscopy, conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, signal and image digital processing. His main interests are molecular oncology (chromatin, endocytosis and adhesion mechanisms), neuroscience (brain mapping and neuronal network signaling) and smart materials (intelligent drug delivery and nanocomposite materials).

(*) http://www.biophysics.org/Membership/Subgroups/NanoscaleBiophysics/tabid/728/Default.aspx (**) https://www.iit.it/images/stories/scientific_plan/iit-strategic_plan_2015-2017.pdf (***) https://scholar.google.com/citations?user=FtRb-LIAAAAJ&hl=it (*V) http://www.biophysics.org/AwardsFunding/SocietyAwards/PastAwardees/tabid/5497/Default.aspx

Microscopy skills

Super resolved fluorescence microscopy, Correlative Nnaoscopy, Mueller matrix signature, Bioimaging.

Confocal and Multiphoton Microscopy, i.e., FRAP, FRET, SHG, lifetime imaging, spectral fingerprint, single molecule detection, colocalization, 3D/4D.

Live imaging down to molecular resolution, single molecule/particle tracking with 5-10 nm accuray, 100-40 nm resolution, single molecule sensitivity, Nanoscopy - FCS, TIRF, STED/PALM, IML-SPIM (Individual Molecule Localization - Selective Plane Illumination Microscopy).

4D (x, y, z, t) particle tracking, fast scanning modes - 100 fps, 512x512 pixel, photoactivatable fluo markers - paGFP, other fluo probes.

Active optical microscopy molecular 3D/4D uncaging and events follow up.

Deep imaging towards small animal live imaging, depth penetration, 0.5-1 mm.

Integration with electrophysiological data, multimodal platform for simultaneous imaging and data analysis.

Implementation of a deconvolution and image processing platform powermicroscope - friendly remote web access, correlative microscopy vs. TEM, SEM, AFM.

25 selected publications

1. Lanzanò L., Coto Hernandez I., Castello M., Gratton E., Diaspro A., Vicidomini G. (2015) “Encoding and decoding spatio-temporal information for super-resolution microscopy” Nature communications.

2. Viero G., Lunelli L., Passerini A., Bianchini P., Gilbert R. J., Bernabò P., Tebaldi T., Diaspro A., Pederzolli C. and Quattrone A. (2015) Three distinct ribosome assemblies modulated by translation are the building blocks of polysome. Journal of Cell Biology , vol. 208, (no. 5), pp. 581-596 DOI: 10.1083/jcb.201406040

3. Chacko JV, Harke B, Canale C, Diaspro A. (2014) Cellular level nanomanipulation using atomic force microscope aided with superresolution imaging. J Biomed Opt. 19(10):105003. doi: 10.1117/1.JBO.19.10.105003

4. Bianchini P, Cardarelli F, Luca MD, Diaspro A, Bizzarri R. (2014) Nanoscale Protein Diffusion by STED-Based Pair Correlation Analysis. PLoS One. 9 (6):e99619.

doi: 10.1371/journal.pone.0099619

5. Duocastella M., Vicidomini G., Diaspro A. (2014) Simultaneous multiplane confocal microscopy using acoustic tunable lenses. Optics Express. 22(16): 19293-19301

6. Vicidomini G, Coto Hernández I, d'Amora M, Cella Zanacchi F, Bianchini P, Diaspro A (2014) Gated CW-STED microscopy: A versatile tool for biological nanometer scale investigation. Methods. 66(2) 124-130. doi:10.1016/j.ymeth.2013.06.029

7. Deschout H., Zanacchi F.C., Mlodzianoski M., Diaspro A., Bewersdorf J., Hess S.T. and Braeckmans K. (2014) Precisely and accurately localizing single emitters in fluorescence microscopy. Nature Methods. 11(3):253-266. DOI: 10.1038/nmeth.2843.

8. Diaspro A. (2013) Taking three-dimensional two-photon excitation microscopy further: Encoding the light for decoding the brain. Microscopy Research Technique. 76(10): 985- 987. doi: 10.1002/jemt.22284.

9. Chacko J.V., Cella Zanacchi F., Diaspro A. (2013) Probing Cytoskeletal Structures by Coupling Optical Superresolution and AFM Techniques for a Correlative Approach. Cytoskeleton 70(11): 729 – 740. doi: 10.1002/cm.21139

10. Harke B., Dallari W., Grancini G., Fazzi D., Brandi F., Petrozza A., Diaspro A. (2013). Polymerization Inhibition by Triplet State Absorption for Nanoscale Lithography. Advanced Materials. 25 (6): 904-909 doi:10.1002/adma.201204141

11. Ronzitti E., Harke B., Diaspro A. (2013) Frequency dependent detection in a STED microscope using modulated excitation light. Optics Express. 21 (1): 210 – 219.DOI: 10.1364/OE.21.000210.

12. Bianchini P., Harke B., Galiani S., Vicidomini G., Diaspro A. (2012) Single wavelength 2PE-STED super-resolution imaging. PNAS 109 (17): 6390 - 6393. www.pnas.org/cgi/doi/10.1073/pnas.1119129109

13. Cella Zanacchi F, Lavagnino Z., Perrone Donnorso M., Del Bue A., Furia L., Faretta M., Diaspro A. (2011) Live-cell 3D superresolution imaging in thick biological samples. Nature Methods. 8 (12): 1047 – 1049. DOI:10.1038/NMETH.1744

14. Bianchini P., Diaspro A. (2008). Three-dimensional (3d) backward and forward second harmonic generation (shg) microscopy of biological tissues. J Biophotonics. 1(6): 443–50.

15. Palamidessi A., Frittoli E., Garré M., Faretta M., Mione M., Testa I., Diaspro A., Lanzetti L., Scita G., Di Fiore P.P. (2008). Endocytic trafficking of rac is required for the spatial restriction of signaling in cell migration.Cell. 134(1): 135–47.

16. Diaspro, A.; Chirico, G. & Collini, M. (2005) Two-photon fluorescence excitation and related techniques in biological microscopy. Q Rev Biophys 38(2), 97--166.

17. Schneider, M.; Barozzi, S.; Testa, I.; Faretta, M. & Diaspro, A. (2005), 'Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. Biophys Journal 89(2), 1346--1352.

18. Diaspro, A.; Silvano, D.; Krol, S.; Cavalleri, O. & Gliozzi, A. (2002), 'Single Living Cell Encapsulation in Nano-organized Polyelectrolyte Shells', Langmuir 18(13), 5047-5050.

19. Chirico, G.; Cannone, F.; Beretta, S.; Baldini, G. & Diaspro, A. (2001), 'Single molecule studies by means of the two-photon fluorescence distribution.', Microsc Res Tech 55(5), 359--364.

20. Diaspro, A.; Annunziata, S. & Robello, M. (2000), 'Single-pinhole confocal imaging of sub-resolution sparse objects using experimental point spread function and image restoration.' Microsc Res Tech 51(5), 464—468

21. Pecere, T.; Gazzola, M. V.; Mucignat, C.; Parolin, C.; Vecchia, F. D.; Cavaggioni, A.; Basso, G.; Diaspro, A.; Salvato, B.; Carli, M. & Palù, G. (2000), 'Aloe-emodin is a new type of anticancer agent with selective activity against neuroectodermal tumors. Cancer Res 60(11), 2800--2804.

22. Diaspro, A.; Bertolotto, M.; Vergani, L. & Nicolini, C. (1991), 'Polarized light scattering of nucleosomes and polynucleosomes--in situ and in vitro studies.', IEEE Trans Biomed Eng July 1991. 38(7): 670--678.

23. Bianco, B. & Diaspro, A. (1989), 'Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing. Cell Biophys 15(3), 189--199.

24. Diaspro, A. & Nicolini, C. A. (1987), 'Circular intensity differential scattering and chromatin-DNA structure. A combined theoretical approach. Cell Biophys 10(1), 45--60.

25. Beltrame, F.; Bianco, B.; Castellaro, G.; Diaspro, A. & Nicolini, C. (1985), 'Quantitative phase contrast optical microscopy of cells', Medical and biological Engineering and Computing 23, 263--264.