Richard N. Day
Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 635 Barnhill Dr., Indianapolis, IN 46202 USA.
Research in my laboratory applies quantitative fluorescence microscopy techniques to visualize the behaviors of proteins inside living cells. Our studies use the combination of molecular biology, biochemistry, and live-cell imaging approaches to determine how specific signal transduction and gene regulatory complexes are assembled. We are using time-resolved fluorescence lifetime imaging microscopy (FLIM) to measure Förster resonance energy transfer (FRET), which enables us to determine how certain disease-causing point mutations can affect the assembly of specific protein complexes. I have over one hundred publications, with extensive experience in live-cell imaging. In addition, I have taught in the annual Cold Spring Harbor Laboratory or Marine Biological Laboratory live-cell imaging courses yearly since 1998, and I am currently the co-organizer for the Workshop on FRET microscopy, held annually at the University of Virginia since 2001.